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Image Search Results
Journal: Neural Regeneration Research
Article Title: Upregulation of CDGSH iron sulfur domain 2 attenuates cerebral ischemia/reperfusion injury
doi: 10.4103/1673-5374.355766
Figure Lengend Snippet: CISD2 overexpression activates the Nrf2/HO-1 pathway in in vivo and in vitro models of CIRI. (A–D) Representative western blots and quantitative evaluations of the effect on CISD2 overexpression on the expression of nuclear-Nrf2 and HO-1 in cerebral tissue following CIRI in mice. (E–H) Representative western blots and quantitative evaluations of the effect of CISD2 overexpression on the expression of nuclear-Nrf2 and HO-1 in OGD/R-induced HT22 cells. Data are represented as the mean ± SEM ( n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). CIRI: Cerebral ischemia/reperfusion injury; CISD2: CDGSH iron sulfur domain 2; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; HO-1: heme oxygenase-1; I/R: cerebral ischemia/reperfusion; Nrf2: nuclear factor erythroid2-related factor 2; OGD/R: oxygen-glucose deprivation/reoxygenation.
Article Snippet: The membranes were blocked, and incubated overnight with primary antibodies CISD2 (rabbit, 1:1000, CST, Boston, MA, USA, Cat# 60758, RRID: AB_943513), xCT (rabbit, 1:2000, Abcam, Cambridge, UK, Cat# ab37185, RRID: AB_778944), GPX4 (rabbit, 1:2000, Cat# ab125066, RRID: AB_10973901, Abcam), transferrin receptor1 (TFR1; rabbit, 1:800, Abcam, Cat# ab84036, RRID: AB_10673794), Nrf2 (rabbit, 1:800, WanLeiBio, Shenyang, China, Cat# WL02135, RRID: AB_2847838), Lamin B1 (mouse, 1:1000, Proteintech, Wuhan, China, Cat# 12987-1-AP, RRID: AB_2721256), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; rabbit, 1:1000, Cat# 60004-1-Ig, RRID: AB_2263076,
Techniques: Over Expression, In Vivo, In Vitro, Western Blot, Expressing